Preparation of GFLV CP gene cDNA Probe Labled by Photosensitive Biotin and Detection of GFLV
长臂光敏生物素标记葡萄扇叶病毒外壳蛋白基因cDNA探针的制备及其在检测上的应用

The cDNA of the GFLV CP gene amplified from clone pGAB5 by PCR were labeled by photo sensitive biotin.The probe sensitivity is 5pg,and the detectable amount of unlabeled GFLV cDNA was 10pg by dot blot hybridization when the biotin labeled cDNA was used as a probe.The results of detection for GFLV by probe and ELISA have positive correlation.The minimum amount of grapevine tissue for detection by probe was 100 times lower than that by ELISA.The detection for grapevine samples is not limited by the seasons and the position of sampling.