%0 Journal Article
%T Preparation of GFLV CP gene cDNA Probe Labled by Photosensitive Biotin and Detection of GFLV<br>长臂光敏生物素标记葡萄扇叶病毒外壳蛋白基因cDNA探针的制备及其在检测上的应用
%A Cao Xiaozhen
%A Zhang Yuman
%A Cai Wenqi
%A Guan Hancheng
%A Mang Keqiang
%A <br>曹肖真
%A 张玉满
%A 蔡文启
%A 管汉成
%A 莽克强
%J 生物工程学报
%D 1999
%I 
%X The cDNA of the GFLV CP gene amplified from clone pGAB5 by PCR were labeled by photo sensitive biotin.The probe sensitivity is 5pg,and the detectable amount of unlabeled GFLV cDNA was 10pg by dot blot hybridization when the biotin labeled cDNA was used as a probe.The results of detection for GFLV by probe and ELISA have positive correlation.The minimum amount of grapevine tissue for detection by probe was 100 times lower than that by ELISA.The detection for grapevine samples is not limited by the seasons and the position of sampling.
%K GFLV CP
%K gene cDNA probe labled by long arm photosenisitive biotin
%K molecular hybridization
%K GFLV detection<br>长臂光敏生物素GFLV外壳蛋白基因cDNA探针，分子杂交，GFLV检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=331360886E78B358BD164448C814257C&yid=B914830F5B1D1078&vid=23CCDDCD68FFCC2F&iid=CA4FD0336C81A37A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0