Effects of Gene Copy Number and Chromosomal Position on the Expression of a Modified HBsAg Gene SA-28 in Yeast
基因拷贝数与染色体位置对酵母表达乙肝病毒融合表面抗原SA-28基因的影响

The effect of gene copy number and chromosomal position on heterologous gene expression in Saccharomyces cerevisae has been tentatively investigated using a modified hepatitis B virus surface antigen SA 28 gene.The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated targeted integration.The expression of SA 28 gene in the resultant transformants was then measured.The result indicated that the expression of SA 28 gene was positively related to the gene dosage at HIS3 locus and had chromosomal polarity at several chromosomal loci.