%0 Journal Article
%T Effects of Gene Copy Number and Chromosomal Position on the Expression of a Modified HBsAg Gene SA-28 in Yeast<br>基因拷贝数与染色体位置对酵母表达乙肝病毒融合表面抗原SA-28基因的影响
%A PAN Hui
%A GAO Xiang
%A Dong
%A WANG Fei
%A YUAN Hang
%A Ying
%A LI Yu
%A Yang
%A <br>潘辉
%A 高向东
%A 王飞
%A 袁汉英
%A 李育阳
%J 生物工程学报
%D 2000
%I 
%X The effect of gene copy number and chromosomal position on heterologous gene expression in Saccharomyces cerevisae has been tentatively investigated using a modified hepatitis B virus surface antigen SA 28 gene.The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated targeted integration.The expression of SA 28 gene in the resultant transformants was then measured.The result indicated that the expression of SA 28 gene was positively related to the gene dosage at HIS3 locus and had chromosomal polarity at several chromosomal loci.
%K Yeast
%K gene copy number
%K chromosomal position effect
%K hepatitis B virus surface antigen
%K FLP recombinase<br>酵母
%K 基因拷贝数
%K 染色体位置效应
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A4B365E71DDEDC69&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=2F56B21F91C9B05B&eid=1F199509C0B6C4D6&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=16