Expression of the CRH Region of G CSF Receptor in Insect Cell
人粒细胞集落刺激因子受体膜外区在昆虫细胞中的表达

The CRH cDNA of G CSF receptor gene was cloned into the plasmid pFASTBAC. After transformed the E.coli DH10BAC, recombinant baculovirus genome which contained CRH cDNA was obtained. By transfecting the insect cell SF 9 with the recombinant baculavirus genome, the recombinant baculavirus was obtained. SDS PAGE and Western blot analysis of the infected cell lysit showed that CRH was expressed in the SF 9 cell. The result of the receptor binding experiment also proved that the expression product purfied by the G CSF affinity column can specifically bind the 125 IG CSF with the Kd of 3.8nmol/L.