%0 Journal Article
%T Expression of the CRH Region of G CSF Receptor in Insect Cell<br>人粒细胞集落刺激因子受体膜外区在昆虫细胞中的表达
%A Zhao Bingwen
%A Li Lei Li Fusheng Chen Aijun Zeng Gefei Zhang Zhiqing Hou Yunde
%A <br>赵炳文
%A 李蕾
%A 李福胜
%A 陈爱君
%A 曾革非
%A 张智清
%A 侯云德
%J 生物工程学报
%D 1999
%I 
%X The CRH cDNA of G CSF receptor gene was cloned into the plasmid pFASTBAC. After transformed the E.coli DH10BAC, recombinant baculovirus genome which contained CRH cDNA was obtained. By transfecting the insect cell SF 9 with the recombinant baculavirus genome, the recombinant baculavirus was obtained. SDS PAGE and Western blot analysis of the infected cell lysit showed that CRH was expressed in the SF 9 cell. The result of the receptor binding experiment also proved that the expression product purfied by the G CSF affinity column can specifically bind the 125 IG CSF with the Kd of 3.8nmol/L.
%K G
%K CSF
%K receptor
%K bacularvirus expressing system
%K purification<br>人粒细胞集落刺激因子，受体，聚合酶链反应，杆状病毒表达系统，亲和层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B0F55D7147DB579CE336F06C4A57FB84&yid=B914830F5B1D1078&vid=23CCDDCD68FFCC2F&iid=E158A972A605785F&sid=4D4C81DBA842B7BD&eid=8F2250DA83AF77B8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=4