Insect-resistant Tobacco Plants Expressing Insect-specific Neurotoxin AaIT
表达昆虫特异性神经毒素AaIT基因的转基因烟草的抗虫性

The recombinant plant expression vector pNGY-2 with designed and synthesized AaIT gene had been constructed. The AalT gene was fused downstream the sequence of TMV and inserted into expression vector under the control of two linked 35S promoters. The recombinant plasmid pNGY-2 was transferred into tobacco NC89 by agrobacterium-mediated leaf-disc gene transger system. The GUS activity analysis and Southern blotting of regenerated plants indicated that AalT gene had been introduced into tobacco genome. Insect bioassays showed that transgenic plants had notable insect-resistant activity.