%0 Journal Article
%T Insect-resistant Tobacco Plants Expressing Insect-specific Neurotoxin AaIT<br>表达昆虫特异性神经毒素AaIT基因的转基因烟草的抗虫性
%A Yao Bin Fan Yunliu Zeng Qin Zhao Rongmin
%A <br>姚斌
%A 范云六
%A 曾勤
%A 赵荣敏
%J 生物工程学报
%D 1996
%I 
%X The recombinant plant expression vector pNGY-2 with designed and synthesized AaIT gene had been constructed. The AalT gene was fused downstream the sequence of TMV and inserted into expression vector under the control of two linked 35S promoters. The recombinant plasmid pNGY-2 was transferred into tobacco NC89 by agrobacterium-mediated leaf-disc gene transger system. The GUS activity analysis and Southern blotting of regenerated plants indicated that AalT gene had been introduced into tobacco genome. Insect bioassays showed that transgenic plants had notable insect-resistant activity.
%K AaIT gene
%K transgenic tobacco plants
%K insect-resistant activity<br>AaIT基因
%K 转基因烟草
%K 抗虫活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC97487C1E58BA99AFDF&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&sid=A63576421B012172&eid=7F5DDA4924737DF5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=2