Preparation and Characterization of Monoclonal Antibodies to Aflatoxin B_1
抗黄曲霉毒素B1单克隆抗体的制备及特性

Five hybridoma cell lines producing monoclonal antibodies (McAb) against aflatoxin B1 (AFB1) were established after fusion of mouse myecloma cells (Sp-2/0-Ag-14) with spleen cells isolated from male BALB/c mice immunized with AFB1-BSA conjugate. Among them, a McAb which was designated AFB1-2H8 was of the subtype IgG3 and the ascitic fluid of it gave suitably high dilution titres (1: 5X106). The sensitivity of a competitive enzyme- (linked immunosorbent assay (ELISA) using AFB1-2H8 for AFB1 was demonstrated that the linear range was 0. 5~50ng/ml and the minimum detectable concentration of AFB1 was 0. 01ng/ml. The specificity of the McAb was determined and it was shown no cross-rection significantly with any of the metabolites tested. So the McAb and the ELISA described may prove of use in the detection of AFB] foods and feeds.