%0 Journal Article
%T Preparation and Characterization of Monoclonal Antibodies to Aflatoxin B_1<br>抗黄曲霉毒素B1单克隆抗体的制备及特性
%A Lu Ge Liu Baoshun Liu Chunxia
%A <br>路戈
%A 刘宝顺
%A 刘春霞
%A 王德斌曹明华
%J 生物工程学报
%D 1995
%I 
%X Five hybridoma cell lines producing monoclonal antibodies (McAb) against aflatoxin B1 (AFB1) were established after fusion of mouse myecloma cells (Sp-2/0-Ag-14) with spleen cells isolated from male BALB/c mice immunized with AFB1-BSA conjugate. Among them, a McAb which was designated AFB1-2H8 was of the subtype IgG3 and the ascitic fluid of it gave suitably high dilution titres (1: 5X106). The sensitivity of a competitive enzyme- (linked immunosorbent assay (ELISA) using AFB1-2H8 for AFB1 was demonstrated that the linear range was 0. 5~50ng/ml and the minimum detectable concentration of AFB1 was 0. 01ng/ml. The specificity of the McAb was determined and it was shown no cross-rection significantly with any of the metabolites tested. So the McAb and the ELISA described may prove of use in the detection of AFB] foods and feeds.
%K Aflatoxin B1
%K monoclonalantibody
%K enzyme-linked immunosorbent assay<br>黄曲霉毒素B1，单克隆抗体，酶联免疫吸附测定法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D958442C697A140980F8745B342A2569&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=E158A972A605785F&sid=5FF9F4F7CB1800C7&eid=556C1A86E372B606&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=10&reference_num=6