Protoplast Isolation from Root Suspension Culture Cells and Plant Regeneration in Rice
从水稻根部悬浮培养细胞分离原生质体及植株再生

After loose calli are produced from tender roots Oryza sativa L. on MS and N6 media, much active protoplasts are obtained by means of protoplasts isolated from callus-derived shock suspension culture in an agitated liquid AA medium. The experiment shows that this is an ideal material to isolate protoplasts . Calli from protoplasts are developed in MS agarose medium containing 2, 4-D Img/L (the following unit is the same ), KT 0. 2, NAA 0. 2, glutamine 867; asparagine 266. Plantlets are induced on N6 medium containing KT 2, NAA 0. 2, CH 1000, 4% coconut milk. The plants generated from protoplast R! generation are able to bloom, produce and seed normally; the number of chromosomes is diploidy (2n = 24). The most characteristic of all is the low rate of the seed setting, reduction in the number grains of each spike and the prolonged time of growth .