%0 Journal Article
%T Protoplast Isolation from Root Suspension Culture Cells and Plant Regeneration in Rice<br>从水稻根部悬浮培养细胞分离原生质体及植株再生
%A Jia Yongjiong Chen Fang Li Ying He Xiaoli
%A <br>贾勇炯
%A 陈放
%A 李英
%A 何小莉
%J 生物工程学报
%D 1994
%I 
%X After loose calli are produced from tender roots Oryza sativa L. on MS and N6 media, much active protoplasts are obtained by means of protoplasts isolated from callus-derived shock suspension culture in an agitated liquid AA medium. The experiment shows that this is an ideal material to isolate protoplasts . Calli from protoplasts are developed in MS agarose medium containing 2, 4-D Img/L (the following unit is the same ), KT 0. 2, NAA 0. 2, glutamine 867; asparagine 266. Plantlets are induced on N6 medium containing KT 2, NAA 0. 2, CH 1000, 4% coconut milk. The plants generated from protoplast R! generation are able to bloom, produce and seed normally; the number of chromosomes is diploidy (2n = 24). The most characteristic of all is the low rate of the seed setting, reduction in the number grains of each spike and the prolonged time of growth .
%K Rice
%K suspension culture of root cell
%K plant generated from protoplast<br>水稻，根部悬浮培养细胞，原生质体再生植株
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DB10256DAFAFE5E2E027F6B65AA2BDA5&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=0B39A22176CE99FB&sid=BBF7D98F9BEDEC74&eid=9D453329DCCABB94&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=5