Cloning and Sequencing of PLRV Coat Protein Gene and Construction of Vectors for.Transgenic Potato Resistant to Coinfection of PVY, PVX and PLRV
马铃薯单双三价抗病毒基因表达载体的构建

The coat protein gene of potato leaf roll virus (Zhangjakou isolate) was cloned by PCR technology and sequenced. Comparisons of the nucleotide and deduced amino acid sequences of the PLRV coat protein gene with those of some reported isolates reveal over 97% homology between them. In order to acquire transgenic potato resistant to coinfection of PVY, PVX and PLRV, we managed to put the three virus coat protein genes driven by the enhanced 35S promoter respectively in one plant expression vector.