%0 Journal Article
%T Cloning and Sequencing of PLRV Coat Protein Gene and Construction of Vectors for.Transgenic Potato Resistant to Coinfection of PVY, PVX and PLRV<br>马铃薯单双三价抗病毒基因表达载体的构建
%A Cui Xiaojiang Peng Xuexian Shen Yufei Mang Keqiang
%A <br>崔晓江
%A 彭学贤
%A 沈禹飞
%A 莽克强
%J 生物工程学报
%D 1994
%I 
%X The coat protein gene of potato leaf roll virus (Zhangjakou isolate) was cloned by PCR technology and sequenced. Comparisons of the nucleotide and deduced amino acid sequences of the PLRV coat protein gene with those of some reported isolates reveal over 97% homology between them. In order to acquire transgenic potato resistant to coinfection of PVY, PVX and PLRV, we managed to put the three virus coat protein genes driven by the enhanced 35S promoter respectively in one plant expression vector.
%K Potato leafroll virus
%K coat protein
%K coinfection
%K transgenic potato<br>马铃薯
%K 抗病毒基因
%K 载体
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0795B99E992E80EEC498EA9AC77230F0&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=0B39A22176CE99FB&sid=D46BA3D3D4B3C585&eid=3A0155B37D8FF829&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=7&reference_num=3