Isolation and Purification of the Fused Protein Encoded by Synthetic Antigen Gene of Plasmodium Falciparum and Its Expression in Escherichia coli
恶性疟合成多肽抗原基因在大肠杆菌中表达产物的分离纯化

The fusion protein from synthesized plasmodium falciparum hybrid antigen gene expressed in E. colt was purified. The recombinants (pWR/A ?7,pWR/B ?164, pWR/AB ?20)grew in Luria broth medium with lactose. The bacteria were harvested by centrifugation and the pelleted cells were, suspended in lysis buffer containing NP-40 and lysozyme and were treated by sonication and centrifugation. The fusion protein was isolated with SDS-PAGE and recovered by electrophoresis. One to four mg pure fusion protein can be obtained from 80-100 ml bacterial culture media. (Rabbits were immunized by the purified fusion protein and serologic assay has demonstrated with immuno-geneicity).