%0 Journal Article
%T Isolation and Purification of the Fused Protein Encoded by Synthetic Antigen Gene of Plasmodium Falciparum and Its Expression in Escherichia coli<br>恶性疟合成多肽抗原基因在大肠杆菌中表达产物的分离纯化
%A Chen Shirong Wang Changcai Zhong Xionglin Hu Yafang Wang Qisong
%A <br>陈仕荣
%A 王昌才
%A 钟雄林
%A 胡亚芳
%A 王启松
%J 生物工程学报
%D 1995
%I 
%X The fusion protein from synthesized plasmodium falciparum hybrid antigen gene expressed in E. colt was purified. The recombinants (pWR/A ?7,pWR/B ?164, pWR/AB ?20)grew in Luria broth medium with lactose. The bacteria were harvested by centrifugation and the pelleted cells were, suspended in lysis buffer containing NP-40 and lysozyme and were treated by sonication and centrifugation. The fusion protein was isolated with SDS-PAGE and recovered by electrophoresis. One to four mg pure fusion protein can be obtained from 80-100 ml bacterial culture media. (Rabbits were immunized by the purified fusion protein and serologic assay has demonstrated with immuno-geneicity).
%K Plasmodium falciparum
%K antigen gene expression
%K protein isolation and purification
%K polyacrylamide gel electrophoresis<br>疟疾
%K 疟原虫
%K 合成多肽
%K 抗原
%K 表达产物
%K 提纯
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A04916AA4E26EED5ACC06F92A56B9266&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=0B39A22176CE99FB&sid=E0F6F365E4766526&eid=FCD27DC5E1F2EEE7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=5