ELECTRON MICROSCOPIC 3D-RECONSTRUCTION OF MOUSE HIPPOCAMPAL PYRAMIDAL DENDRITIC SPINES
小鼠海马锥体细胞树突棘形态的电镜三维重建

The complex three-dimensional (3D) shapes of most neurons are difficult to observe. With laser scanning confocal microscopy, the shapes of labeled neurons in three dimensions can be reconstructed rapidly, but identification of subtle structures requires electron microscopy. Through serial sections electron microscopy and computer-based SD-reconstruction technique subtle 3D structure of neurons can be obtained. EM 3D-reconstruction technique was introduced, and the key procedures and skills were emphasized. Using this technique, the dendritic spines of pyramidal cell in hippocampus of both young and mature mice were analyzed. The results provide important information for the study of structure, function and plasticity of dendritic spines.