%0 Journal Article
%T ELECTRON MICROSCOPIC 3D-RECONSTRUCTION OF MOUSE HIPPOCAMPAL PYRAMIDAL DENDRITIC SPINES<br>小鼠海马锥体细胞树突棘形态的电镜三维重建
%A YANG Guang
%A GONG Kai
%A JIANG Wu-ling
%A GONG Yan-dao
%A ZHANG Xiu-fang
%A ZHAO Nan-ming
%A <br>杨光
%A 龚锴
%A 蒋五玲
%A 公衍道
%A 张秀芳
%A 赵南明
%J 生物物理学报
%D 2003
%I 
%X The complex three-dimensional (3D) shapes of most neurons are difficult to observe. With laser scanning confocal microscopy, the shapes of labeled neurons in three dimensions can be reconstructed rapidly, but identification of subtle structures requires electron microscopy. Through serial sections electron microscopy and computer-based SD-reconstruction technique subtle 3D structure of neurons can be obtained. EM 3D-reconstruction technique was introduced, and the key procedures and skills were emphasized. Using this technique, the dendritic spines of pyramidal cell in hippocampus of both young and mature mice were analyzed. The results provide important information for the study of structure, function and plasticity of dendritic spines.
%K Transmission electron microscope
%K Serial ultrathin sections
%K 3D-reconstruction
%K Dendritic spine<br>透射电子显微镜
%K 连续超薄切片
%K 三维重建
%K 树突棘
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=8625B1F6512DABDC&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=13553B2D12F347E8&eid=BC12EA701C895178&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=3&reference_num=15