The Application of Homology Modeling in The Cellulase Molecule Evolution
同源建模在纤维素酶分子改造中的应用

Homology modeling methodology has inspired much new hope for the researches of protein, it solves problems in the protein structure prediction, functional analysis and protein engineering theoretically. Cellulases are increasingly being more important for a large variety of industrial purposes, e.g. within the fuel industry, in the textile industry, in paper pulp processing and so on. Consequently substantial effort has gone into cellulase's cloning, expression and site-directed mutagenesis as well as their structures, functions, and interactions of components of cellulase enzyme systems. However, the reported three-dimensional structures of cellulases from Protein Date Base are not sufficient to the rational design of cellulase mutants, so homology modeling methodology has been applied into molecule artificial evolution of cellulase. Its application mainly includes the mechanism study of functional amino acids, rational design of mutant sites, family homology analysis, structural prediction and functional study of mutants. Along with the development of molecular docking and molecular dynamics simulation, homology modeling will display powerful vitality in the cellulase molecule evolution.