DAMAGE TO MEMBRANE LIPIDS AND PROTEINS OF BRAIN MICROSOMES BY HYDROXYL RADICALS
羟基自由基对兔脑微粒体膜脂及膜蛋白的损伤

The effect of hydroxyl radical on the lipid peroxidation and (Na + K)-ATPase activity of rabbit brain microsomes has been studied. Results show that hydroxyl radicals induce the lipid peroxidation and increase the formation of malondialdehyde. Incubation of microsomes with hydroxyl radical-generation system results in loss of SH groups and inhibition of (Na + K)-ATPase activity. Addition of sodium ferulate inhibits the lipid peroxidation, effectively protects (Na + K)-ATPase activity and reduces the loss of SH groups. Spin trapping experiments show that sodium ferulate markedly scavenges the hydroxyl radicals.