MICRO CARBON FIBER ELECTRODE FOR RECORDING OF QUANTAL SECRETION IN SINGLE CELL
微碳纤电极与细胞量子化分泌的记录方法

Biophysical technologies for single cell recording was described, with special focus on micro carbon fiber electrode (CFE) and its application in stimulus-secretion coupling. In contrast to membrane capacitance of patch-clamp recording, CFE has advantages in high sensitivity, real detection of release substance and no need for tight-seal patch-clamp, although it detects only a few easily oxidizable transmitters including catecholamines and serotonin. In combination with patch-clamping and Ca imaging measurement, coupling between action-potentials and quantal transmitter secretion in chromaffin cells are demonstrated as the example of CFE application.