%0 Journal Article
%T MICRO CARBON FIBER ELECTRODE FOR RECORDING OF QUANTAL SECRETION IN SINGLE CELL<br>微碳纤电极与细胞量子化分泌的记录方法
%A HE Li-ming
%A DUAN Kai-lai
%A ZHANG Chen
%A HE Lin-ling
%A XIONG Wei
%A LI Jie
%A ZHOU Zhuan
%A <br>何立铭
%A 段开来
%A 张晨
%A 何林玲
%A 熊巍
%A 李洁
%J 生物物理学报
%D 2002
%I 
%X Biophysical technologies for single cell recording was described, with special focus on micro carbon fiber electrode (CFE) and its application in stimulus-secretion coupling. In contrast to membrane capacitance of patch-clamp recording, CFE has advantages in high sensitivity, real detection of release substance and no need for tight-seal patch-clamp, although it detects only a few easily oxidizable transmitters including catecholamines and serotonin. In combination with patch-clamping and Ca imaging measurement, coupling between action-potentials and quantal transmitter secretion in chromaffin cells are demonstrated as the example of CFE application.
%K Secretion
%K Patch-clamp
%K Neurotransmitter
%K Membrane capacitance
%K Carbon fiber electrode<br>微碳纤电极
%K 细胞量子化分泌
%K 记录方法
%K 细胞分泌
%K 膜片钳
%K 神经递质
%K 膜电容
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=AA89781AF226835A&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=0B39A22176CE99FB&sid=2922B27A3177030F&eid=7CE3F1F20DE6B307&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=33