CLONING AND CHARACTERIZATION OF THE GENE(ahr) ENCODING ATP-DEPENDENT DNA HELICASE REP FROM VIBRIO ALGINOLYTICUS STRAIN HY9901
溶藻弧菌HY9901 ahr基因克隆与序列分析

A 1.2 kb internal fragment of the ATP-dependent DNA helicase rep gene from Vibrio alginolyticus strain HY9901 was amplified by PCR with designed primers and the unknown flanking sequences of 5'-and 3'-ends of the ahr gene were characterized by using inverse PCR and nested PCR. Sequence analysis showed the ahr gene contained a 2016 bp ORF encoding 671 amino acids. Compared with uther relevant vibrios,the deduced amino acid sequence of the ATP-dependent DNA helicase rep precursor from Vibrio alginolyticus strain HY9901 possesses very high similarity with them.