%0 Journal Article
%T CLONING AND CHARACTERIZATION OF THE GENE(ahr) ENCODING ATP-DEPENDENT DNA HELICASE REP FROM VIBRIO ALGINOLYTICUS STRAIN HY9901<br>溶藻弧菌HY9901 ahr基因克隆与序列分析
%A CAI Shuang-Hu
%A WU Zao-He
%A JIAN Ji-Chang
%A LU Yi-Shan
%A <br>蔡双虎
%A 吴灶和
%A 简纪常
%A 鲁义善
%J 水生生物学报
%D 2008
%I 
%X A 1.2 kb internal fragment of the ATP-dependent DNA helicase rep gene from Vibrio alginolyticus strain HY9901 was amplified by PCR with designed primers and the unknown flanking sequences of 5'-and 3'-ends of the ahr gene were characterized by using inverse PCR and nested PCR. Sequence analysis showed the ahr gene contained a 2016 bp ORF encoding 671 amino acids. Compared with uther relevant vibrios,the deduced amino acid sequence of the ATP-dependent DNA helicase rep precursor from Vibrio alginolyticus strain HY9901 possesses very high similarity with them.
%K Vibrio alginolyticus
%K ATP-dependent DNA helicase rep gene
%K Gene cloning<br>溶藻弧菌
%K 依赖ATP的DNA解旋rep基因
%K 基因克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=41D3B1C5ABA76F6A83BD5D9CDC194399&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=E158A972A605785F&sid=CA4FD0336C81A37A&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=0&reference_num=13