THE EFFECT OF MODIFICATION OF SER AND LYS RESIDUES ON THE STRUCTURE AND FUNCTION OF BACTERIORHODOPSIN(BR)
菌紫质的Ser和Lys残基修饰对BR结构和功能的影响

Chemical modification was used tO study the structure and function of BR.Lysine and serine residues were modified with free radical modifier S and modifier F.Compared with CD spectrum of normal purple membrane, for the samples treated with free radical modifier S and F respectively,24 hours after modification, 535nm positive peak disappeared, and negative peaks at 596nm and 602nm remain. 72 hours after modification, 535nm positive peak reappeared,and no further change was observed later than 120 hours after modification. Compared with unmodified BR, for the samples of two modified BR, the quantity of M412 increaseed. The effect of two kinds of modifier on the change of structure and function of BR were discussed.