%0 Journal Article
%T THE EFFECT OF MODIFICATION OF SER AND LYS RESIDUES ON THE STRUCTURE AND FUNCTION OF BACTERIORHODOPSIN(BR)<br>菌紫质的Ser和Lys残基修饰对BR结构和功能的影响
%A Hu Kunsheng
%A Wang Aojin
%A Sun Yongtai
%A <br>胡坤生
%A 王敖金
%A 孙永泰
%J 生物物理学报
%D 1996
%I 
%X Chemical modification was used tO study the structure and function of BR.Lysine and serine residues were modified with free radical modifier S and modifier F.Compared with CD spectrum of normal purple membrane, for the samples treated with free radical modifier S and F respectively,24 hours after modification, 535nm positive peak disappeared, and negative peaks at 596nm and 602nm remain. 72 hours after modification, 535nm positive peak reappeared,and no further change was observed later than 120 hours after modification. Compared with unmodified BR, for the samples of two modified BR, the quantity of M412 increaseed. The effect of two kinds of modifier on the change of structure and function of BR were discussed.
%K Bacteriorhodopsin
%K Chemical modification
%K M_(412) intermediate<br>菌紫质，化学修饰，M＿（412）中间体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=BDBED1B2AB7109112165259AEEB872F5&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&sid=717CC18E05F2AFA0&eid=9DC563A0FEFC04F9&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=3