DNA damage and alteration of expression in apoptosis related proteins induced by okadaic acid in FL cells
大田软海绵酸对FL细胞DNA的损伤及凋亡相关蛋白表达的影响

The objective of this work was to evaluate deoxyribonucleic acid(DNA) damage and expression of Bcl-2,Bax,p53 proteins in okadaic acid-treated human amniotic FL cells.Cells were cultured for 4 h with 0,20,40,60,80 and 100 nmol okadaic acid(OA),or 100 nmol OA for periods of 2,4 and 8 h.Comet assay results indicated that DNA damage was a dose-dependent increase and western blot analysis showed that the expression of Bcl-2,Bax,p53 proteins was time-and dose-dependent decrease.Therefore,it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis,and on the contrary, Bax and p53 were related to the proliferation of FL cell.