%0 Journal Article
%T DNA damage and alteration of expression in apoptosis related proteins induced by okadaic acid in FL cells<br>大田软海绵酸对FL细胞DNA的损伤及凋亡相关蛋白表达的影响
%A XING Ming-Luan
%A LOU Jian-Lin
%A XU Li-Hong
%A <br>邢鸣鸾
%A 楼建林
%A 徐立红
%J 水生生物学报
%D 2007
%I 
%X The objective of this work was to evaluate deoxyribonucleic acid(DNA) damage and expression of Bcl-2,Bax,p53 proteins in okadaic acid-treated human amniotic FL cells.Cells were cultured for 4 h with 0,20,40,60,80 and 100 nmol okadaic acid(OA),or 100 nmol OA for periods of 2,4 and 8 h.Comet assay results indicated that DNA damage was a dose-dependent increase and western blot analysis showed that the expression of Bcl-2,Bax,p53 proteins was time-and dose-dependent decrease.Therefore,it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis,and on the contrary, Bax and p53 were related to the proliferation of FL cell.
%K Bcl-2
%K Bax
%K p53<br>大田软海绵酸
%K DNA损伤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=62D675891C8A3AF52586E2A5D39C392F&yid=A732AF04DDA03BB3&vid=4AD960B5AD2D111A&iid=94C357A881DFC066&sid=CA4FD0336C81A37A&eid=D559883475316B44&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=0&reference_num=12