STUDIES OF RELATIONS OF GLUTAMATE UPTAKE BY ASTROCYTE WITH ENVIROMENT FACTORS AND ION TRANSPORT
星形神经胶质细胞摄取谷氨酸与环境因子和离子运输的关系

Astrocyte cultures and radioisotopic tracer method were used in this study. The effects of ions (Na-, K+, Cl-), of different concentration of kainic acidand of some inhibitors in media on uptake of L-glutamate and influence of L-glutamate uptake on Na+, K+, Cl-, Ca2+ ion transport on cell membrane were explored. The results suggest that the uptake of L-glutamate depended on the concentration of Na + . Also, it depended obviously on concentration of Cl- in the absence of Na+ in media, but not in the pre-sence of phsiological concentration of Na + . The glutamate uptake was decreased when K- concentration was increased which caused depolarization. On the other hand, glutamate uptake stimulated the transports of Na+, K+, Cl-. The results also show that uptake of glutamate was inhibited by 10-3 or 10-2 M Kainic acid and the inhibitors used.