In VitroBioactivity of Collagen/Calcium Phosphate Silicate Composites, Cross-Linked with Chondroitin Sulfate

In this work we present the experimental results on synthesis structure and in vitro bioactivity of collagen (C)/calcium phosphate silicate (CPS)/chondroitin sulfate (ChS) composites with C/CPS ratio of 25:75 wt. % and 75:25wt. %. The synthesized composites were characterized by XRD, FTIR and SEM/EDS. XRD and FTIR before in vitro test proved that the carbonate containing hydroxyapatite (CO3HA) formed in situ. After in vitro test for 3 days in 1.5 SBF, the nucleation of B-type CO3HA was estimated on the obtained C/CPS=75:25 wt. % composite with cauliflower-like assembly. On the C/CPS=25:75 wt. % amorphous hydroxyapatite (HA) layer was observed.