Archaeal acylamino acid releasing enzyme/lipase: Crystallization and preliminary crystallographic analysis in a new crystal form

A primitive orthorhombic crystal form of acylamino acid releasing enzyme/lipase (APE1547) from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291 K. The diffraction pattern of the crystal extends to 0.27 nm resolution at 100 K using Cu Kα radiation. The crystal belongs to the space group P212121 with unit cell dimensions of a = 6.399, b = 10.439 and c = 16.953 nm. The presence of two molecules per asymmetric unit gives a crystal volume per protein mass (Vm) of 0.0022 nm3 Da 1 and a solvent content of 43% by volume. A full set of X-ray diffraction data were collected to 0.3 nm from the native crystal.