%0 Journal Article
%T Archaeal acylamino acid releasing enzyme/lipase: Crystallization and preliminary crystallographic analysis in a new crystal form
%A Ganggang Wang
%A Renjun Gao
%A Haitao Yang
%A Shugui Cao
%A Yan Feng
%A Zihe Rao
%J Chinese Science Bulletin
%@ 1861-9541
%D 2003
%I 
%R 10.1360/03tb9031
%X A primitive orthorhombic crystal form of acylamino acid releasing enzyme/lipase (APE1547) from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291 K. The diffraction pattern of the crystal extends to 0.27 nm resolution at 100 K using Cu K¦Á radiation. The crystal belongs to the space group P212121 with unit cell dimensions of a = 6.399, b = 10.439 and c = 16.953 nm. The presence of two molecules per asymmetric unit gives a crystal volume per protein mass (Vm) of 0.0022 nm3 Da 1 and a solvent content of 43% by volume. A full set of X-ray diffraction data were collected to 0.3 nm from the native crystal.
%K AARE
%K Aeropyrum. pernix
%K orthorhombic crystal
%K asymmetric unit
%U http://link.springer.com/article/10.1360/03tb9031