Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P< 0.05). The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.