%0 Journal Article
%T Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology
%A Wenwei Hu
%A Yingnian Yu
%A Xingruo Chen
%A Tao Song
%A Haiyang Xie
%J Chinese Science Bulletin
%@ 1861-9541
%D 1999
%I 
%R 10.1007/BF02885542
%X Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N¡ä-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P< 0.05). The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.
%K antisense RNA
%K mutation
%K nontargeted
%K genetic instability
%U http://link.springer.com/article/10.1007/BF02885542