|
Detección de Poliovirus en Aguas Residuales de Armenia, ColombiaDOI: 10.1590/S0124-00642006000400002 Keywords: sewage, polymerase chain reaction, poliovirus, colombia. Abstract: objectiveimplementing environmental surveillance for poliovirus circulating from wastewater. methods six wastewater samples were collected from each final site within a wastewater collection system in armenia, quindío. virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and sabin vaccine-specific primers. results it was confirmed that the viral detection method is highly efficient. poliovirus was detected in 5 of 6 (83%) samples collected. serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. all of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country. conclusion wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
|