%0 Journal Article
%T Detecci¨®n de Poliovirus en Aguas Residuales de Armenia, Colombia
%A Gonz¨¢lez
%A Mar¨ªa M
%A Sarmiento
%A Lu¨ªs
%A Casta£¿o
%A Jhon Carlos
%A Giraldo
%A Alejandra M
%A Salazar
%A Alexander
%A Mu£¿oz
%A Nini J
%J Revista de Salud P¨²blica
%D 2006
%I Universidad Nacional de Colombia
%R 10.1590/S0124-00642006000400002
%X objectiveimplementing environmental surveillance for poliovirus circulating from wastewater. methods six wastewater samples were collected from each final site within a wastewater collection system in armenia, quind¨ªo. virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and sabin vaccine-specific primers. results it was confirmed that the viral detection method is highly efficient. poliovirus was detected in 5 of 6 (83%) samples collected. serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. all of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country. conclusion wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
%K sewage
%K polymerase chain reaction
%K poliovirus
%K colombia.
%U http://www.scielosp.org/scielo.php?script=sci_abstract&pid=S0124-00642006000400002&lng=en&nrm=iso&tlng=en