Obtención de un conjugado látex inmunoglobulina para el diagnóstico de Gardnerella vaginalis

to obtain an anti-gardnerella vaginalis latex globuline, specific immunosera to reference strain (atcc 14018) and to a clinical isolation strain pool were produced. these inmunosera were characterized using page-sds and inmunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. the latex globuline conjugates obtained from these 2 inmunosera were evaluated in vitro with a resulting level of detection of 10 ufc/ml of gardnerella vaginalis. these two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. the anti-strain pool (ii) latex globuline conjugate turned out to be more sensitive and specific than anti-g. vaginalis atcc strain (i) latex globuline.