%0 Journal Article
%T Obtenci¨®n de un conjugado l¨¢tex inmunoglobulina para el diagn¨®stico de Gardnerella vaginalis
%A Espinosa
%A Ivette
%A ¨¢lvarez
%A Elba
%A Amaral
%A Cintia
%A Alonso
%A Magaly
%A Lorenzo
%A Mait¨¦
%J Revista Cubana de Medicina Tropical
%D 2000
%I Centro Nacional de Informaci¨®n de Ciencias M¨¦dicas
%X to obtain an anti-gardnerella vaginalis latex globuline, specific immunosera to reference strain (atcc 14018) and to a clinical isolation strain pool were produced. these inmunosera were characterized using page-sds and inmunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. the latex globuline conjugates obtained from these 2 inmunosera were evaluated in vitro with a resulting level of detection of 10 ufc/ml of gardnerella vaginalis. these two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. the anti-strain pool (ii) latex globuline conjugate turned out to be more sensitive and specific than anti-g. vaginalis atcc strain (i) latex globuline.
%K gardnerella vaginalis [isolation and purification]
%K vaginosis
%K bacterial [diagnosis]
%K latex fixation tests.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07602000000200004&lng=en&nrm=iso&tlng=en