Evaluación de la transcripción del locus rfb para la biosíntesis de LPS en Leptospira interrogans subtipo Hardjoprajitno

plasmid pgl4w15-96 was constructed from the pgl4w74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed jl5 promoter with the objective of confirming the role of j15 sequence as a promoter for leptospira, which restored the transcription of the gene of resistance to kanamycin in escherichia coli and leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.