%0 Journal Article
%T Evaluaci¨®n de la transcripci¨®n del locus rfb para la bios¨ªntesis de LPS en Leptospira interrogans subtipo Hardjoprajitno
%A Carrillo Casas
%A Erika Margarita
%A Rojano R¨ªos
%A Raquel
%A Bulach
%A Dieter
%A Adler
%A Ben
%A de la Pe£¿a-Moctezuma
%A Alejandro
%J Revista Cubana de Medicina Tropical
%D 2005
%I Centro Nacional de Informaci¨®n de Ciencias M¨¦dicas
%X plasmid pgl4w15-96 was constructed from the pgl4w74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed jl5 promoter with the objective of confirming the role of j15 sequence as a promoter for leptospira, which restored the transcription of the gene of resistance to kanamycin in escherichia coli and leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.
%K leptsopira
%K lps
%K promoter
%K plasmid.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07602005000100008&lng=en&nrm=iso&tlng=en