Resultados preliminares del perfeccionamiento del DAVIH-AgP24 con el empleo del sistema de amplificación biotina-tiramina/estreptavidina-peroxidasa

objective: elisa davih-ag p24 was modified by introducing heat dissociation of plasma samples and a tyramine/streptavidine-peroxidase amplification system, with the objective of increasing sensitivity. methods: between-assay repeatability was determined in amplified davih-ag p24. thirty two plasma samples from hiv-1-infected individuals classified in three clinical categories (aids case, asymptomatic and minor opportunistic infections) were evaluated. results: the variation coefficient ranged 4-10.3 % in between-assay repeatability. with the amplified davih-p24 ag, the p24 antigen detection level increased to 0.5 pg/ml. amplified davih-p24 ag reached 66 % sensitivity whereas standard davih-p24 ag sensitivity rate was 31 %. conclusions: this preliminary study proved that the introduction of new modifications in amplified davih-p24 ag managed to increase the p24 antigen detection levels and to gain sensitivity.