%0 Journal Article
%T Resultados preliminares del perfeccionamiento del DAVIH-AgP24 con el empleo del sistema de amplificaci¨®n biotina-tiramina/estreptavidina-peroxidasa
%A Fragas Quintero
%A Anitza
%A Ortiz Losada
%A Eva
%A D¨ªaz Torres
%A H¨¦ctor M.
%A Silva Cabrera
%A Eladio
%A Izquierdo M¨¢rquez
%A Maricela
%J Revista Cubana de Medicina Tropical
%D 2009
%I Centro Nacional de Informaci¨®n de Ciencias M¨¦dicas
%X objective: elisa davih-ag p24 was modified by introducing heat dissociation of plasma samples and a tyramine/streptavidine-peroxidase amplification system, with the objective of increasing sensitivity. methods: between-assay repeatability was determined in amplified davih-ag p24. thirty two plasma samples from hiv-1-infected individuals classified in three clinical categories (aids case, asymptomatic and minor opportunistic infections) were evaluated. results: the variation coefficient ranged 4-10.3 % in between-assay repeatability. with the amplified davih-p24 ag, the p24 antigen detection level increased to 0.5 pg/ml. amplified davih-p24 ag reached 66 % sensitivity whereas standard davih-p24 ag sensitivity rate was 31 %. conclusions: this preliminary study proved that the introduction of new modifications in amplified davih-p24 ag managed to increase the p24 antigen detection levels and to gain sensitivity.
%K p24 antigen
%K immune complex dissociation
%K biotin-tyramine/streptavidine-peroxidase amplification system
%K antiretroviral therapy.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07602009000100002&lng=en&nrm=iso&tlng=en