Establecimiento de una técnica para la determinación de enteroparásitos en vegetales mediante inmunofluorescencia

there are biological risks associated with vegetables including the human pathogen parasites, thus it was necessary to establish a technique to assessment of entero-parasites in these foods. this procedure was applied according to the method described in the fda bacteriological analysis manual / cfsan of 2001 where ten vegetable samples were investigated from five culture areas from the ciudad de la habana province from january to march, 2009. authors used a direct examination with concentrated lugol's solution and the immunofluorescence technique for giardia and cryptosporidium by monoclonal antibodies kit (merifluor c/g). for control of parasites extraction process we took a sample of lettuce adding a suspension based in giardia cysts. by direct examination we achieved the 14,4% of recovery and by immunofluorescence the 57,8%. results obtained for immunofluorescence control were the expected ones. in two assay samples there were giardia cysts by immunofluoresence but not cryptosporidium cysts.