%0 Journal Article
%T Establecimiento de una t¨¦cnica para la determinaci¨®n de enteropar¨¢sitos en vegetales mediante inmunofluorescencia
%A Puig Pe£¿a
%A Yamila
%A Carrera Vara
%A Jos¨¦
%A Leyva Castillo
%A Virginia
%A Hern¨¢ndez Castro
%A Idalmis
%J Revista Cubana de Higiene y Epidemiolog£¿-a
%D 2011
%I Scientific Electronic Library Online
%X there are biological risks associated with vegetables including the human pathogen parasites, thus it was necessary to establish a technique to assessment of entero-parasites in these foods. this procedure was applied according to the method described in the fda bacteriological analysis manual / cfsan of 2001 where ten vegetable samples were investigated from five culture areas from the ciudad de la habana province from january to march, 2009. authors used a direct examination with concentrated lugol's solution and the immunofluorescence technique for giardia and cryptosporidium by monoclonal antibodies kit (merifluor c/g). for control of parasites extraction process we took a sample of lettuce adding a suspension based in giardia cysts. by direct examination we achieved the 14,4% of recovery and by immunofluorescence the 57,8%. results obtained for immunofluorescence control were the expected ones. in two assay samples there were giardia cysts by immunofluoresence but not cryptosporidium cysts.
%K parasite
%K vegetables
%K immunofluorescence
%K giardia
%K cryptosporidium.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1561-30032011000100004&lng=en&nrm=iso&tlng=en