Ensayo inmunoenzimático para la determinación de anticuerpos naturales anti banda 3

an immunoenzimatic assay (elisa) was standardized to determine the natural anti-band 3 antibodies by coupling with chemobond plates the band 3 protein obtained from the membrane of human erythrocytes by a combination of affinity and anion exchange chromatography. 3 g of band 3 protein per well were used. a level of detection of 1 g/ml of anti-band 3 antibody was achieved. the results showed the usefulness of the method to measure the levels of natural anti-band 3 antibodies in patients with drepanocytes, both in basal state and in the painful vasocclusive crises.