%0 Journal Article
%T Ensayo inmunoenzim¨¢tico para la determinaci¨®n de anticuerpos naturales anti banda 3
%A Arce Hern¨¢ndez
%A Ada Amalia
%A Villaescusa Blanco
%A Rinaldo
%A Merl¨ªn Linares
%A Julio C¨¦sar
%J Revista Cubana de Hematolog£¿-a, Inmunolog£¿-a y Hemoterapia
%D 2008
%I Editorial Ciencias M¨¦dicas
%X an immunoenzimatic assay (elisa) was standardized to determine the natural anti-band 3 antibodies by coupling with chemobond plates the band 3 protein obtained from the membrane of human erythrocytes by a combination of affinity and anion exchange chromatography. 3 g of band 3 protein per well were used. a level of detection of 1 g/ml of anti-band 3 antibody was achieved. the results showed the usefulness of the method to measure the levels of natural anti-band 3 antibodies in patients with drepanocytes, both in basal state and in the painful vasocclusive crises.
%K natural anti-band 3 antibodies
%K drepanocytosis
%K elisa
%K band 3 protein.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0864-02892008000300006&lng=en&nrm=iso&tlng=en