Estandarización y validación de una metodología analítica para la determinación de carbamazepina en plasma mediante extracción en fase sólida y cromatografía líquida de alta eficiencia

this paper describes the analytical results during validation of a reverse high performance liquid chromatography methodology for the assay of carbamazepine in human plasma, useful for their dosage in biodisponibility and bioequivalence studies. the stationary bounded phase used was octadecylsilane and the mobile phase was composed by a mixture of methanol-acetonitrile-water. the detection was performed by uv at 213 nm and lorazepam was utilized as internal standard. the method show linearity between 125 ng and 10 mcg/ml, amounts useful for the assay of carbamazepine in plasma.