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Apr 02, 2015Open    Access

Comparative Optimized Protocols of DNA Extraction and Purification Using FTA PlantSaver Card and DNAzol Methods for Eggplant (Solanum Species) Studies in North Central Nigeria

Celestine Uzoma Aguoru, Lucky O. Omoigui, Joseph Olalekan Olasan
Two methods of DNA extraction and purification in eggplant molecular research were investigated. FTA PlantSaver card and DNAzol were optimized, simplified and presented. The aim was to discard the rigorous protocols and help save energy, resources and time wasted in DNA extraction during molecular research in eggplant. DNA was extracted from fresh leaf of 18-day-old eggplant seedlings. Quality of genomic DNA extracts was verified on 1% agarose gel electrophoresis and viewed on a UV-transillumina...
Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101406


Mar 13, 2015Open    Access

Investigation of the Effects of Eucalyptus camaldulensis on Performance of Neighbouring Crop Productivity in Western Amhara, Ethiopia

Melkamu Alebachew, Tadele Amare, Minale Wendie
The study was conducted on investigating effects of Eucalyptus camaldulensis on the performance of adjacent crop productivity for the benefits over adverse effects of it versus different land uses in district Mecha of Western Amhara region, Ethiopia. This research was carried out with the aim to test the performance of neighbouring crops grown near E. camaldulensis on the effects on plant growth performance and grain yield. Eucalypts has many important uses and a reliable source of...
Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1100992


Mar 04, 2015Open    Access

Evaluation of Synergistic Effect of Neem and Poultry Manure on Root Knot Nematode (Meloidogyne spp.) Infecting Rice

Hassan Mohammed Auwal, Ibrahim Buba Galadima, Jacob Madu, Paul Joseph

Field experiment was conducted in 2014 to test the efficacy of different organic amendments on the control of root-knot nematode (Meloidogyne spp.). The different materials are sole neem, sole poultry manure, and a combination of neem and poultry manure. Neem was applied at the rate of 160 g per plot, poultry manure was applied at the rate of 1120 g per plot, combination of neem and poultry manure was applied at 1280 g per plot and Furadan was applied at 1

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Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101372


Jan 27, 2015Open    Access

Potential Impact of Climate Change on Dynamics of Coffee Berry Borer (Hypothenemus hampi Ferrari) in Ethiopia

Eshetu Agegnehu, Ashok Thakur, Tewodros Mulualem
The aim of this paper is to review the potential effect of climate change on dynamics of coffee berry borer and to generate information for researches concerned on it. Coffee (Coffea arabica L.), which is originated in Ethiopia, is the backbone of the country’s economy. It accounts for 70% of the foreign exchange earning, 10% of the government revenue and employs 25% of the domestic labor force. There are four coffee production systems in Ethiopia such as modern plantation, gardens, semi-...
Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101127


Jan 26, 2015Open    Access

Medicinal Plants in the Ethno Veterinary Practices of Bensa Woreda, Southern Ethiopia

Yibrah Tekle

Ethno botanical study on traditional medicinal plants were conducted between November, 2013 and September, 2014 in Bensa Woreda, Southern Ethiopia and documented different types of traditional medicinal plants used by the indigenous peoples. The study was focused on identifying medicinal plants, plant habit, disease treated, plant parts used, way of knowledge acquired, methods of preparation, route of administration, importance/indication, and ingredients added.

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Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101258


Jan 26, 2015Open    Access

Evaluation of Garlic (Allium sativum L.) Varieties for Bulb Yield and Growth at Dabat, Northwestern Ethiopia

Asrat Ayalew, Daniel Tadesse, Zenebe G. Medhin, Solomon Fantaw
A field experiment was conducted for two consecutive years (2013 and 2014) during the dry periods under irrigation at Dabat District, Northwestern Ethiopia to select the best adaptable and high yielder varieties. Five garlic varieties (BishoftuNech, Kuriftu, Tseday 92, MM-98 and the local) were planted on randomized complete block design with three replications. Data on days to emer- gence, days to maturity, pseudo stem height, number of leaves per plant, bulb diameter, number of cloves per bulb...
Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101216


Jan 07, 2015Open    Access

Cotton Flea Beetle, Podagrica puncticollis Weise (Coleoptera: Halticidae) in Metema District, Northern Ethiopia: Yield Loss Assessment and Management

Eshetu Agegnehu Abebe
Flea beetles are the most destructive insect pests of cotton in Northwestern part of Ethiopia. Damage is the most severe when seedlings are attacked and may necessitate extensive resowing. A field experiment was carried out in Metema districtfrom June to December in 2007 cropping season to estimate yield losses due to cotton flea beetle and to determine the efficacy of seed dressing and foliar insecticides against Podagrica puncticollis. The treatments were arranged by using RCBD with thr...
Open Access Library J.   Vol.2, 2015
Doi:10.4236/oalib.1101128


Dec 22, 2014Open    Access

Development of Molecular Marker and Variability Characterization of Aspergillus flavus Isolates of Chilies (Capsicum frutescens L.) through RAPD-PCR and Estimation of Aflatoxin B1 by Indirect Competitive ELISA in India

Virendra Kumar, Ajay Kumar, Shailesh Kumar Kannojia, Rana Pratap Singh
Aflatoxin is the most potent toxic substances, these are the group of closely related mycotoxins produced by fungus i.e. Aspergillus flavus. Twenty seven isolates of A. flavus designated as Af-1, Af-2, Af-4, Af-5, Af-7, Af-9, Af-11, Af-12, Af-13, Af-15, Af-25, Af-28, Af-31, Af-33, Af-34, Af-35, Af-36, Af-37, Af-39, Af-40, Af-46, Af-47, Af-54, Af-55, Af-58, Af-59 and Af-60 were collected from different agro climatic conditions from chilies (Capsicum frutescence) samples. Random amplified polymorp...
Open Access Library J.   Vol.1, 2014
Doi:10.4236/oalib.1101161


Nov 27, 2014Open    Access

Role of Lead on Chlorophylls and Soluble Proteins in Some Cultivars of Triticum aestivum L. under Osmotic Potential

K. A. Farghali, Afnan S. A. Quronfulah

The changes of chlorophylls and main metabolites in three cultivars of Triticum aestivum L. under the effects of osmotic and lead stresses, as well as their interaction, were investigated. The results indicated that, the decreased Ψs and Pb concentration caused an increase in the chlorophyll content in all plants. Conversely, the chl. a/b ratio was decreased. Apparently, the total soluble proteins were increased in response to low

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Open Access Library J.   Vol.1, 2014
Doi:10.4236/oalib.1101097


Oct 30, 2014Open    Access

Flowering and Seed Development in Onion—A Review

Khalid Mahmud Khokhar
Proper vernalization temperature of mother onion bulbs stimulates early flowering and produces a heavier yield of seed. Moreover, onion cultivars seem to differ in their optimal temperature requirements depending on the locality to which they are adapted. Most studies on the effects of storage temperature on inflorescence initiation in onion have shown that the optimum temperature for flowering is in a range between 5℃ to 13℃ for 90 to 120 days and bolting resistant varieties require a longer co...
Open Access Library J.   Vol.1, 2014
Doi:10.4236/oalib.1101049


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