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Biological Procedures Online

ISSN Print: 1480-9222

ISSN Online:

Website: http://www.biologicalproceduresonline.com

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A new method of kidney biopsy using low dose CT-guidance with coaxial trocar and bard biopsy gun

Pi Xiao-ling,Tang Zhen,Fu Li-qian,Guo Mei-hua

A do-it-yourself protocol for simple transcription activator-like effector assembly

Uhde-Stone Claudia,Gor Nilang,Chin Tiffany,Huang Joseph

Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods

Sedlackova Tatiana,Repiska Gabriela,Celec Peter,Szemes Tomas

Determine the quality of human embryonic stem colonies with laser light scattering patterns

Chen Chi-Shuo,Biasca Matthew,Le Catherine,Chen Eric Y-T

An improved competitive inhibition enzymatic immunoassay method for tetrodotoxin quantification

Amber N Stokes, Becky L Williams, Susannah S French

A reinvestigation of somatic hypermethylation at the PTEN CpG island in cancer cell lines

Luke B Hesson, Deborah Packham, Emily Pontzer, Pauline Funchain, Charis Eng, Robyn L Ward

Novel mouse mammary cell lines for in vivo bioluminescence imaging (BLI) of bone metastasis

Celeste M Bolin, Caleb Sutherland, Ken Tawara, Jim Moselhy, Cheryl L Jorcyk

A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

David C McMullen, William S Kean, Ajay Verma, Jeffrey T Cole, William D Watson

A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Erin L Symonds, Michael Fenech

Enhancement of anti-murine colon cancer immunity by fusion of a SARS fragment to a low-immunogenic carcinoembryonic antigen

Chen-Si Lin, Shih-Han Kao, Yu-Cheng Chen, Chi-Han Li, Yuan-Ting Hsieh, Shang-Chih Yang, Chang-Jer Wu, Ru-Ping Lee, Kuang-Wen Liao

Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

Michael Flanagan, Jeffrey M Gimble, Gang Yu, Xueqing Xia, Bruce A Bunnell, Shulin Li

Modified protocol for in vivo imaging of wild-type mouse retina with customized miniature spectral domain optical coherence tomography (SD-OCT) device

Ferguson Lee R,Balaiya Sankarathi,Grover Sandeep,Chalam Kakarla V

A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors

Uhde-Stone Claudia,Huang Joseph,Lu Biao

Spontaneous arthritis and ankylosis in male DBA/1 mice: further evidence for a role of behavioral factors in “stress-induced arthritis”

Braem Kirsten,Carter Shea,Lories Rik J

A quantitative PCR method for measuring absolute telomere length

Nathan J O'Callaghan, Michael Fenech

Quantitative Assessment of Mammary Gland Density in Rodents Using Digital Image Analysis

John N McGinley, Henry J Thompson

A rapid and easy method for the DNA extraction from Cryptococcus neoformans

Fatma Mseddi, Mohammed Jarboui, Amira Sellami, Hayet Sellami, Ali Ayadi

Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

Vladimir N Noskov, Nicholas CO Lee, Vladimir Larionov, Natalay Kouprina

An automated cell-counting algorithm for fluorescently-stained cells in migration assays

Baraa K Al-Khazraji, Philip J Medeiros, Nicole M Novielli, Dwayne N Jackson

Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes

Cecilia Mancini, Erika Messana, Emilia Turco, Alessandro Brussino, Alfredo Brusco

Frequency shifting approach towards textual transcription of heartbeat sounds

Farshad Arvin, Shyamala Doraisamy, Ehsan Safar Khorasani

Media composition influences yeast one- and two-hybrid results

Ying Liu, Zabeena Merchant, Hao-Ching Hsiao, Kim L Gonzalez, Kathleen S Matthews, Sarah E Bondos

Biological Procedures Online now publishing with BioMed Central

Shulin Li

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

Chen Gang,Kronenberger Peter,Teugels Erik,De Grève Jacques

Highly Efficient Stable Expression of Indoleamine 2,3 Dioxygenase Gene in Primary Fibroblasts

Alireza Rezakhanlou, Darya Habibi, Amy Lai, Reza B Jalili, Christopher J Ong, Aziz Ghahary

A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

Holger Weishaupt, Joanne L Attema

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

Gang Chen, Peter Kronenberger, Erik Teugels, Jacques De Grève

Optimized RNA Extraction and Northern Hybridization in Streptomycetes

Marcello Tagliavia, Anna Taravella, Sandra Marineo, Anna Puglia, Mario La Farina

Quick and Simple Detection Technique to Assess the Binding of Antimicrotubule Agents to the Colchicine-Binding Site

Sébastien Fortin, Jacques Lacroix, Marie-France C？té, Emmanuel Moreau, éric Petitclerc, René C-Gaudreault

Utilization of IκB–EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity

Yu-Ling Lin, Yen-Shun Chen, Jui-Hung Hsieh, Ching-Min Lin, Hsin-Yi Wu, Chen-Si Lin, Rea-Min Chu, Kuang-Wen Liao, Yuan-Hsun Hsu

Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis

John N McGinley, Zongjian Zhu, Weiqin Jiang, Henry J Thompson

A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis

John N McGinley, Matthew D Thompson, Henry J Thompson

A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors

Yu-Ling Lin, Yun-Ju Lai, Nu-Man Tsai, Tai-Chu Peng, Yen-Ku Liu, Ru-Ping Lee, Chueh-Jen Tsai, Kuang-Wen Liao

Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

Claudio Muscari, Chiara Gamberini, Ilaria Basile, Francesca Bonafé, Simond Valgimigli, Ombretta Capitani, Carlo Guarnieri, Claudio Caldarera

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Mandal Nakul,Heegaard Steffen,Prause Jan,Honoré Bent

A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

Weishaupt Holger,Attema Joanne

Highly Efficient Stable Expression of Indoleamine 2,3 Dioxygenase Gene in Primary Fibroblasts

Rezakhanlou Alireza,Habibi Darya,Lai Amy,Jalili Reza

Quick and Simple Detection Technique to Assess the Binding of Antimicrotubule Agents to the Colchicine-Binding Site

Fortin Sébastien,Lacroix Jacques,C？té Marie-France,Moreau Emmanuel

Utilization of IκB–EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity

Lin Yu-Ling,Chen Yen-Shun,Hsieh Jui-Hung,Lin Ching-Min

Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

Muscari Claudio,Gamberini Chiara,Basile Ilaria,Bonafé Francesca

Optimized RNA Extraction and Northern Hybridization in Streptomycetes

Tagliavia Marcello,Taravella Anna,Marineo Sandra,Puglia Anna

A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors

Lin Yu-Ling,Lai Yun-Ju,Tsai Nu-Man,Peng Tai-Chu

Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis

McGinley John,Zhu Zongjian,Jiang Weiqin,Thompson Henry

A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis

McGinley John,Thompson Matthew,Thompson Henry

A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf

Wu Ning,Matand Kanyand,Williams Sonya

Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs

Anita Gothelf, Pernille Hojman, Julie Gehl

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Nakul Mandal, Steffen Heegaard, Jan Prause, Bent Honoré, Henrik Vorum

SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease

Lau Wai-Hoe, Leong Wing-Seng, Zhari Ismail, Gam Lay-Harn

The Possible Cellular Mechanism for Extending Lifespan of Mice with Rapamycin

Shulin Li

Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1

Andrea Star？íchová, Luká？ Kubala, Eva Lincová, Zuzana Pernicová, Alois Kozubík, Karel Sou？ek

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