【Objective】 To clarify the species of stem nematode from diseased sample of Codonopsis pilosula in Gansu Province. 【Method】 Species identification of stem nematode was carried out by combing morphological analysis, phylogenetic analyses of ITS-rDNA and 28S-rDNA sequences, PCR with species-specific primers as well as PCR-ITS-RFLP.【Result】 The morphological characteristics of the stem nematode population from C. pilosula in Gansu was similar to those of Ditylenchus arachis. The ranges of measureme...

Agricultural Science  Parasitology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202111034

The soybean cyst nematode (SCN), Heterodera glycines, is the most damaging pathogen of soybeans in the United States. To assess the severity of nematode infestations in the field, SCN egg population densities are determined. Cysts (dead females) of the nematode must be extracted from soil samples and then ground to extract the eggs within. Sucrose centrifugation commonly is used to separate debris from suspensions of extracted nematode eggs. We present a method using OptiPrep as a density gradie...

Veterinary Medicine  Taxonomy  Automata  Agricultural Engineering  Environmental Sciences  Environmental Sciences  Animal Behavior  Agricultural Science  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Electric Engineering  Industrial Engineering  Computer Engineering  Biochemistry  Biodiversity  Bioengineering  Geology  Hydrology  Parasitology  Plant Science  Soil Science  Synthetic Biology  Agronomy 

Doi:10.1371/journal.pone.0223386

Soybeans are an important crop for global food security. Every year, soybean yields are reduced by numerous soybean diseases, particularly the soybean cyst nematode (SCN). It is difficult to visually identify the presence of SCN in the field, let alone its population densities or numbers, as there are no obvious aboveground disease symptoms. The only definitive way to assess SCN population densities is to directly extract the SCN cysts from soil and then extract the eggs from cysts and count the...

Electric Engineering  Computer Engineering  Ecosystem Science  Technology  Biodiversity  Agricultural Engineering  Bioengineering  Environmental Sciences  Artificial Intelligence  Agricultural Science  Biological Engineering  Food Science & Technology  Parasitology  Plant Science  Soil Science  Agronomy 

Doi:10.1038/s41598-021-82261-w

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobia...

Ecology  Developmental Biology  Agricultural Engineering  Environmental Sciences  Artificial Intelligence  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Diagnostics  Biological Materials  Electric Engineering  Genetic Engineering  Industrial Engineering  Computer Engineering  Health Policy  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology  Infectious Diseases  Immunology  Internal Medicine  Metabolic Sciences  Medical Genetics  Allergy & Clinical Immunology  Zoology  Biological Chemistry  Applied Physics  Taxonomy  Medicinal Chemistry  Agricultural Science  Analytical Chemistry  Mycology  Public Health  Mathematical Biology  Marine Biology  Molecular Biology  Microbiology  Global Health  Parasitology  Plant Science  Translational Medicine  Soil Science  Synthetic Biology  Materials Engineering 

Doi:10.1016/j.ohx.2021.e00249

Population-scale and rapid testing for SARS-CoV-2 continues to be a priority for several parts of the world. We revisit the in vitro technology platforms for COVID-19 testing and diagnostics—molecular tests and rapid antigen tests, serology or antibody tests, and tests for the management of COVID-19 patients. Within each category of tests, we review the commercialized testing platforms, their analyzing systems, specimen collection protocols, testing methodologies, supply chain logistics, and rel...

Allergy & Clinical Immunology  Electrochemistry  Information Technologies  Medical Education  Biological Chemistry  Virology  Medicinal Chemistry  Developmental Biology  Environmental Sciences  Biological Engineering  Genomics  Genetics  Food Science & Technology  Marketing  Oncology  Technology And Innovation  Theoretical Chemistry  Diagnostics  Supply Chain Management  Biological Materials  Nanometer Materials  Drugs & Devices  Public Health  Organic Chemistry  Epidemiology  Sales Management  Molecular Biology  Health Policy  Microbiology  Global Health  Bioengineering  Physical Chemistry  Bioinformatics  Biotechnology  Functional Materials  Computational Biology  Cell Biology  Parasitology  Translational Medicine  Infectious Diseases  Women’s Health  Immunology  Respiratory Medicine  Materials Engineering  Internal Medicine  Metabolic Sciences  Medical Genetics 

Doi:10.3390/s21196581

Garcinia husor is one of the folk medicines in Maluku-Indonesia. This species has been used for the treatmet of Malaria disease. The phytochemical contents and antiplasmodial activity not reported yet. In this study we evaluated the quantitative phytochemicals, in vitro and in vivo antiplasmodial activity of stem bark ethyl acetate extract. In vitro assay was done using P. falciparum 3D7 strain sensitive of chloroquine. For in vivo analysis, four groups of M. musculus were infected by P. berghei...

Pharmacology  Organic Chemistry  Parasitology 

Doi:10.4308/hjb.26.2.81

Many new facts and theories have appeared in physiology. The solution of complex physiological problems required collaboration of physiologists, pathologists, biochemists, biophysicists, mathematicians, and other specialists. That was possible due to the rapid development of new technologies as well as the robust foundation laid by the outstanding physiologists of the 19th century. This article describes the activities of outstanding Russian and Ukrainian physiologists of the 19th and the early...

Mathematical Biology  Parasitology  Biochemistry  Developmental Biology  Biophysics 

Doi:10.18523/2617-4529.2018.34-42

Twenty-two Laudakia caucasia (Agamidae) from Iran were examined for helminths and found to harbor 3 species of Nematoda: Skrjabinodon pigmentatus, Spauligodon lacertae, and Thelandros baylisi. Laudakia caucasia represents a new host record for Sk. pigmentatus and Sp. lacertae; Iran is a new locality record for all 3 nematode species. Thelandros baylisi had the highest prevalence of infection (91%) followed by Sk. pigmentatus (45%) and Sp. lacertae (36%).

Zoology  Parasitology  Ecology  Ecosystem Science  Biodiversity  Environmental Sciences  Environmental Sciences 

Doi:http://dx.doi.org/10.1654/4537.1

Eighteen lesser mouse-eared bats, Myotis blythii (Tomes 1857) (Vespertilionidae) from Zanjan Province, Iran, were examined for parasites and found to harbor one species of Cestoda, Hymenolepis rhinopomae; one species of Nematoda, Molinostrongylus alatus; one species of Diptera, Penicillidia dufourdi; and one species of mite, Spinturnix andegavinus. Myotis blythii represents a new host record for Hymenolepis rhinopomae, and Iran is a new locality record for Molinostrongylus alatus.

Animal Behavior  Parasitology  Ecology  Ecosystem Science  Biodiversity  Evolutionary Studies  Paleontology  Environmental Sciences  Environmental Sciences 

Doi:http://dx.doi.org/10.1654/4627.1

ABSTRACT Background: Incidences of sudden deaths (SD) are increasing day by day, especially in younger people. During routine practice, forensic persons have to deal not only criminal, accidental and suicidal deaths, but a large amount of cases of sudden deaths also. Most of the sudden deaths of younger victims are unexpected, clinically unexplained or otherwise obscure. Aims & Objectives: The present study was undertaken to know the major contributory causes of death in young victims of sudde...

Neuroscience  Biological Chemistry  Virology  Biotechnology  Toxicology  Microbiology  Cell Biology  Parasitology  Anthropology  Biochemistry  Entomology